The Effect of Thawing Duration and Different Temperatures in the Vitality of Semen Preserved by Freezing for Five Years in Holstein Bulls

Author(s): Ayser Hamid Salman, Ahmed Alsalihi and Bashar Adham Ahmed

Abstract:

This study was carried out in the artificial insemination center of the Department of Livestock in the Abu Ghraib region/Ministry of Agriculture (25 km west of Baghdad) and the Animal Physiology Laboratory at the College of Agriculture, University of Diyala in February 2024, using sperm preserved by freezing for five years from five Holstein bulls, ages ranging from 2.5-3 years old and with a body weight ranging between 500-750 kg/bull. The experiment was divided into three treatments: T1, thawing straws of frozen semen at a temperature of 37°C and a thawing duration of 30 seconds. T2, thawing straws of frozen semen at a temperature of 50°C and a thawing duration of 12 seconds. T3, thawing straws of frozen semen at a temperature of 60°C and a thawing duration of 6 seconds. The characteristics were studied after preservation by freezing for five years (percentage of individual motility, live and dead sperm,%, integrity of the plasma membrane and acrosome,%, percentage of deformities in the head, midsection, and tail, total deformities, and percentage of normal sperm), where the results showed in all the characteristics studied the moral and mathematical superiority of the first treatment, T1, compared to With the second transaction T2 and the third transaction T3. On the other hand, the second treatment, T2, excelled morally and mathematically compared to the third treatment, T3.

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