Chromatographic Characterizations of Bioactive Compounds in Barleria Prionitis

Author(s): Dr. Shanta Belani

Abstract:

The number of people suffering from renal disorders is increasing manifold every year. It is cause of concern all over the world. This research was planned as herbal medicines are safe and their potential for human consumption is quite high. Study of plant Barleria prionitis followed by soxhlet extraction method using the different solvents like ethanol, methanol, and chloroform. The qualitative chemical investigation was carried out with the crude extract of leaves of B. prionitis and the phytoconstituents were identified by performing various chemical tests, which demonstrated the presence of various phytoconstituents. Representative Silica TLC separations and Column chromatography of B. prionitis extracts was carried out using different solvent systems. The different fractions of phytoconstituents are further characterized by HPTLC. Separation of extract in chloroform, Rf value is 0.744, resulted in bands. Similarly in methanol extract max Rf value is 0.8 and also seen in ethanol extract max Rf value of 0.849. Specific compounds identified in plant samples by HPTLC include tannins, alkaloids, and flavonoids. For instance, peaks at Rf values of 0.01 (Tannin 1) and 0.39 (Gallic acid) suggest the presence of tannins. Alkaloids are indicated by peaks at Rf values of 0.40 (Chelidonine), 0. 0.53 (Alkaloid 1), 0.69 or 0.70 (Alkaloid 2) indicates the presence of alkaloids The peaks at Rf values 0.33 (quercetin-3-O-rutinoside), 0.39 (Flavonoid 2), 0.64 (quercetin-3-O-glucopyranoside) 0.73 (quercetin-3-O-rhamnoside) and 0.90 (flavanol-o- glycoside) indicates the presence of flavonoids.

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